ReefTox 1, acute on 1 hard SPS coral species
Acute coral ecotoxicity test of a complete cosmetic formulation on Montipora digitata, a hard SPS coral relevant for Indo-Pacific, Caribbean, and Mediterranean reef ecosystems. First-line, publishable live-coral ecotox data.
What is ReefTox 1?
ReefTox 1 is an acute coral ecotoxicity test of a complete cosmetic formulation on one hard SPS coral species, Montipora digitata. The formulation is held in contact with live coral for 96 hours, at a single 50 mg/l realistic-exposure concentration with 6 replicates and 6 blanks. It documents observational acute endpoints on living coral tissue (bleaching, tissue loss, necrosis, mortality), the entry-level, first-line live-coral test, documented in a defensible coral ecotox report. Because it uses a single concentration, it does not produce EC50, LOEC or NOEC. Every engagement begins with the free predictive pre-evaluation, which routes the formulation to the compatible panel before any coral is exposed.
Methodology
Protocol
96-hour contact
Coral species
1 species, Montipora digitata (hard SPS)
Concentration
1 concentration at 50 mg/l (realistic-exposure standard)
Replicates
6 replicates + 6 blanks
Acute turnaround is 2 to 3 weeks.
Endpoints
What does ReefTox 1 report?
ReefTox 1 documents observational acute endpoints: bleaching (computer-assisted), tissue loss, necrosis, mortality, morphological / behavioural anomalies (polyp debilitation, mesenteric filament ejection), and before / after photography. Because it uses a single concentration (50 mg/l), it does not produce EC50, LOEC or NOEC.
Who ReefTox 1 is for
- • Brands needing first-line marine ecotox validation for a specific formulation.
- • Regulatory affairs managers building an environmental safety dossier for Hawaii, US Virgin Islands, Palau, or EU compliance.
- • Brands seeking defensible coral ecotox data through live-coral acute testing.
Defensible coral ecotox data
ReefTox 1 is part of the live-coral testing that produces a defensible, publishable coral ecotox report. Every engagement begins with the free predictive pre-evaluation, which routes your formulation to the compatible panel.
Frequently asked questions
What is ReefTox 1?
ReefTox 1 is an acute coral ecotoxicity test of a complete cosmetic formulation on one hard SPS coral species, Montipora digitata. The formulation is held in contact with live coral for 96 hours, at a 50 mg/l realistic-exposure concentration with 6 replicates and 6 blanks. It is the entry-level, first-line live-coral test, documented in a defensible coral ecotox report.
What does ReefTox 1 report?
Observational acute endpoints documenting the coral response: bleaching (computer-assisted), tissue loss, necrosis, mortality, morphological and behavioural anomalies, and before/after photography across the exposure window. Because the test uses a single concentration (50 mg/l), it does not produce EC50, LOEC or NOEC.
How is ReefTox 1 different from the free pre-evaluation?
The free predictive pre-evaluation is an upstream scoring methodology, six formulation pillars plus ICP-OES water analysis and ingredient screening, run before any coral is exposed, to route the formulation to a compatible panel. ReefTox 1 is the live-coral acute test itself. Every engagement begins with the free pre-evaluation; ReefTox 1 follows once it clears the formulation for the panel.
First-line ecotox data, in 2 to 3 weeks.
